Journal:

Article Title: Distribution and Molecular Characterization of Porphyromonas gingivalis Carrying a New Type of fimA Gene

doi:

Figure Lengend Snippet: Comparison of predicted amino acid sequences for FimAs encoded by the fimA genes of various P. gingivalis strains and type V fimA gene. Amino acid identities are shown by asterisks. Hyphens are used to indicate the positions of gaps in the multiple alignment. The putative signal peptides are underlined. The number of amino acids and the molecular weight of the FimA of each strain are given. The alignment of the deduced amino acid sequences was performed with the CLUSTAL W program of the DNA Data Bank of Japan.

Article Snippet: The multiple alignment analysis showed that this gene fragment has sequence homology with type I fimA (strain ATCC 33277) (49%), type II fimA (strain HW24D1) (49%), type III fimA (strain 6/26) (52%), and type IV fimA (strain HG564) (59%).

Techniques: Comparison, Molecular Weight

Journal:

Article Title: Distribution and Molecular Characterization of Porphyromonas gingivalis Carrying a New Type of fimA Gene

doi:

Figure Lengend Snippet: Evolutionary relationships based on synonymous site variation in the fimA gene of P. gingivalis. The neighbor-joining method was used to construct the phylogenetic tree, using CLUSTAL W (DNA Data Bank of Japan) and TreeView software (http://taxonomy.zoology.gla.ac.uk/rod/treeview.html).

Article Snippet: The multiple alignment analysis showed that this gene fragment has sequence homology with type I fimA (strain ATCC 33277) (49%), type II fimA (strain HW24D1) (49%), type III fimA (strain 6/26) (52%), and type IV fimA (strain HG564) (59%).

Techniques: Construct, Software

Journal:

Article Title: Distribution and Molecular Characterization of Porphyromonas gingivalis Carrying a New Type of fimA Gene

doi:

Figure Lengend Snippet: Distribution of P. gingivalis with type I to V fimA in 73 clinical samples from periodontitis patients

Article Snippet: The multiple alignment analysis showed that this gene fragment has sequence homology with type I fimA (strain ATCC 33277) (49%), type II fimA (strain HW24D1) (49%), type III fimA (strain 6/26) (52%), and type IV fimA (strain HG564) (59%).

Techniques:

Journal:

Article Title: Distribution and Molecular Characterization of Porphyromonas gingivalis Carrying a New Type of fimA Gene

doi:

Figure Lengend Snippet: Southern blot analyses of P. gingivalis specific genes. Genomic DNA was isolated from each strain; digested with EcoRI, BamHI, and HindIII; and then separated on a 1% agarose gel. After blotting to a nylon membrane, P. gingivalis specific genes were probed with 32P-labeled fimA, sod, and kgp gene fragments from strain ATCC 33277. Lanes: 1, ATCC 33277; 2, HW24D1; 3, 6/26; 4, HG564; 5, HNA-99.

Article Snippet: The multiple alignment analysis showed that this gene fragment has sequence homology with type I fimA (strain ATCC 33277) (49%), type II fimA (strain HW24D1) (49%), type III fimA (strain 6/26) (52%), and type IV fimA (strain HG564) (59%).

Techniques: Southern Blot, Isolation, Agarose Gel Electrophoresis, Membrane, Labeling

Journal:

Article Title: Distribution and Molecular Characterization of Porphyromonas gingivalis Carrying a New Type of fimA Gene

doi:

Figure Lengend Snippet: Western blot analyses of the FimAs in five type-representative strains of P. gingivalis. Whole-cell lysates of P. gingivalis (2 × 107 cells) were separated by SDS–12% polyacrylamide gel electrophoresis. After electrophoresis, gels were transferred to polyvinylidene difluoride membranes and FimA was detected with antibodies to rFimA (381, type I fimA [A], and HG564, type IV fimA [B]). Lanes: M, prestained protein marker; 1, P. gingivalis ATCC 33277; 2, P. gingivalis HW24D1; 3, P. gingivalis 6/26; 4, P. gingivalis HG564; 5, P. gingivalis HNA-99. Arrows indicate FimA.

Article Snippet: The multiple alignment analysis showed that this gene fragment has sequence homology with type I fimA (strain ATCC 33277) (49%), type II fimA (strain HW24D1) (49%), type III fimA (strain 6/26) (52%), and type IV fimA (strain HG564) (59%).

Techniques: Western Blot, Polyacrylamide Gel Electrophoresis, Electrophoresis, Marker

Journal:

Article Title: Distribution and Molecular Characterization of Porphyromonas gingivalis Carrying a New Type of fimA Gene

doi:

Figure Lengend Snippet: Binding of P. gingivalis representing the five different fimA types to HA and sHA beads. Three milligrams of HA beads equilibrated with buffered KCl or clarified whole human saliva was added to a siliconized borosilicate tube and incubated with different numbers of the 3H-labeled P. gingivalis (106 to 108) cells in a total volume of 300 μl with a gentle, oscillating motion for 1 h at room temperature. The mixture was layered on 100% Percoll to separate unbound cells from the bead-bound cells. After washing, radioactivity of the bead-bound cells was quantitated with a liquid scintillation counter. The results are shown as the mean values of triplicate samples from three individual experiments. One-way analysis of variance and the Tukey-Kramer test were used for the comparison of the binding abilities of P. gingivalis cells (∗, P < 0.0001).

Article Snippet: The multiple alignment analysis showed that this gene fragment has sequence homology with type I fimA (strain ATCC 33277) (49%), type II fimA (strain HW24D1) (49%), type III fimA (strain 6/26) (52%), and type IV fimA (strain HG564) (59%).

Techniques: Binding Assay, Incubation, Labeling, Gentle, Radioactivity, Comparison

Journal: Clinical and Experimental Immunology

Article Title: Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke

doi: 10.1111/cei.13430

Figure Lengend Snippet: Detection of serum IgG antibody titers to periodontal pathogens in favorable and unfavorable groups after stroke

Article Snippet: Sonicated preparations of the following periodontal pathogens were used as bacterial antigens in the present study: Porphyromonas gingivalis ATCC33277 (fimA type I), HW24D1 (fimA type II), 6/26 (fimA type III), W83 (fimA type IV) and HNA99 (fimA type V); Aggregatibacter actinomycetemcomitans ATCC29523 (serotype a), Y4 (serotype b) and AUNY67 (serotype c); Prevotella intermedia ATCC26511, P. nigrescens ATCC33563; Fusobacterium nucleatum ATCC25586 (subspecies nucleatum ) and ATCC 10953 (subspecies polymorphum ); Treponema denticola ATCC35405; Tannerella forsythensis ATCC43037; Campylobacter rectus ATCC33238; and Eikenella corrodens ATCC23834.

Techniques:

Journal: Clinical and Experimental Immunology

Article Title: Serum immunoglobulin G antibody titer to Fusobacterium nucleatum is associated with unfavorable outcome after stroke

doi: 10.1111/cei.13430

Figure Lengend Snippet: Multivariate logistic regression analysis to identify predictive factors for unfavorable outcome following stroke

Article Snippet: Sonicated preparations of the following periodontal pathogens were used as bacterial antigens in the present study: Porphyromonas gingivalis ATCC33277 (fimA type I), HW24D1 (fimA type II), 6/26 (fimA type III), W83 (fimA type IV) and HNA99 (fimA type V); Aggregatibacter actinomycetemcomitans ATCC29523 (serotype a), Y4 (serotype b) and AUNY67 (serotype c); Prevotella intermedia ATCC26511, P. nigrescens ATCC33563; Fusobacterium nucleatum ATCC25586 (subspecies nucleatum ) and ATCC 10953 (subspecies polymorphum ); Treponema denticola ATCC35405; Tannerella forsythensis ATCC43037; Campylobacter rectus ATCC33238; and Eikenella corrodens ATCC23834.

Techniques: